3P008 Mass spectrometry on H/D exchange of poly-L-glutamic acid in helix form
نویسندگان
چکیده
منابع مشابه
pH-jump induced -helix folding of poly-l-glutamic acid
pH jumps are a truly biomimetic technique to initiate non-equilibrium dynamics of biomolecules. In this work, the pH jump induced alpha-helix folding of poly-L-glutamic acid is investigated upon proton release from o-nitrobenzaldehyde. The aim of this work is twofold: On the one hand, design criteria of pH jump experiments are discussed, on the other hand, the folding mechanism of poly-L-glutam...
متن کاملThe effect of aliphatic alcohols on the helix-coil transition of poly-L-ornithine and poly-L-glutamic acid.
The helical stability of poly-L-glutamic acid and poly-Lornithine in aqueous alcohol solutions was studied by optical rotatory dispersion and potentiometric titration determinations. It was found that aliphatic alcohols enhance the stability of the helix according to the series methanol < ethanol < I-propanol < I-butanol. The role of secondary and tertiary alcohols was also investigated. Thermo...
متن کاملEquilibrium unfolding of the poly(glutamic acid)20 helix.
The equilibrium structural ensemble of a 20-residue polyglutamic acid peptide (E(20)) was studied with FRET, circular dichroism, and molecular dynamics (MD) simulations. A FRET donor, o-aminobenzamide, and acceptor, 3-nitrotyrosine, were introduced at the N- and C-termini, respectively. Circular dichroism, steady state FRET, and time-resolved FRET measurements were employed to characterize the ...
متن کاملPoly (L-glutamic acid)-Paclitaxel Conjugates for Cancer Treatment
One of the effective approaches to develop new anticancer drugs is to prepare polymeranticancer drug conjugates. The polymer-anticancer drug conjugates include polymerprotein conjugates, polymer-drug conjugates and supramolecular drug-delivery systems. In 1975, the concept of a polymer-drug conjugate was first proposed by Ringsdorf (Ringsdorf, 1975). In his model, a bioactive anticancer agent w...
متن کاملUV Resonance Raman Determination of Polyproline II, Extended 2.51-Helix, and -Sheet Ψ Angle Energy Landscape in Poly-L-Lysine and Poly-L-Glutamic Acid
UV resonance Raman (UVR) spectroscopy was used to examine the solution conformation of poly-L-lysine (PLL) and poly-L-glutamic acid (PGA) in their non-R-helical states. UVR measurements indicate that PLL (at pH ) 2) and PGA (at pH ) 9) exist mainly in a mixture of polyproline II (PPII) and a novel left-handed 2.51-helical conformation, which is an extended -strand-like conformation with Ψ ≈ +17...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2004
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.44.s191_4